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KMID : 0620920020340030243
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Experimental & Molecular Medicine
2002 Volume.34 No. 3 p.243 ~ p.249
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Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2
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In Kyung Kim/Sang Gun Ahn*
Ho Shik Kim*/Seong Whan Jeong*/Bo Eun Kim*/Hyang Shuk Rhim†/Jae Yong Shim‡/Jin Woo Kim†/Jeong Hwa Lee*/In Kyung Kim*,†
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Abstract
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We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Δ12-PGJ2 induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca2+ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA2 and Δ12-PGJ2 in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.
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KEYWORD
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Sox-4, PGA2, Δ12-PGJ2, apoptosis,
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